Insertion junction: CLIP2.013649_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.013649
Chromosome:chromosome 17
Location:2326518
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g713650 MSH5 DNA mismatch repair protein, MutS homolog; (1 of 1) K08741 - DNA mismatch repair protein MSH5 (MSH5) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCCAATGACGCGACGGAGTTACTTACCGATACCCACCATTTTACCGAG
Internal bar code:GGGGAGAATCGGCACTGCTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1755
LEAP-Seq percent confirming:82.9787
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACACACACACACCT
Suggested primer 2:AGGAAGGGGAGGAGGAAGAC