Insertion junction: CLIP2.013684_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.013684
Chromosome:chromosome 14
Location:517512
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g611300 COP3,CHR1,CSOA (1 of 7) PF01036 - Bacteriorhodopsin-like protein (Bac_rhodopsin); Channelrhodopsin 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCTGCTCATCGTGTCTCCTGTAAAGGTCCGCTGATGATACCCGAATCA
Internal bar code:TTCATAATGGGCCCTTCTAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3019
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:TGACGAGGACGATACAGGGT
Suggested primer 2:CGCAAGGGTGTCGAAGTACT