| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.014110 |
| Chromosome: | chromosome 16 |
| Location: | 7056315 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674627 | MKS1,B9D1 | (1 of 1) K16744 - B9 domain-containing protein 1 (B9D1); B9 Domain-Containing transition zone protein 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGCAGCAGAGGGGGCACGCAGCCGCACACACGCTCGCTCATTCCACC |
| Internal bar code: | TGTTATAGACCTAGCCAGTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2879 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATATGCGCCGTTTGTCCTG |
| Suggested primer 2: | AGTAGAAACGAGACGCGCAA |