| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.014279 |
| Chromosome: | chromosome 2 |
| Location: | 1636717 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085257 | COP4,CHR2,CSOB | Channelrhodopsin 2; (1 of 1) IPR001425//IPR029730 - Archaeal/bacterial/fungal rhodopsin // Archaeal/bacterial/fungal rhodopsin-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAGTGCCACCAATGTTCAATTTGGTGGTCTTGCGAATGTCGCCGTGGA |
| Internal bar code: | TTAAGCCTGACATAAGCTATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5506 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 238 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 238 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATACCGTAGAAGGCCACG |
| Suggested primer 2: | CGTTCATGCCGTTCATTCCC |