| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.014605 |
| Chromosome: | chromosome 6 |
| Location: | 1396759 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g259600 | VPS53 | Subunit of GARP complex; (1 of 1) PTHR12820//PTHR12820:SF0 - VACUOLAR SORTING PROTEIN 53 // VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 53 HOMOLOG | 5'UTR |
| Cre06.g259650 | MPA7 | Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) PTHR10161 - TARTRATE-RESISTANT ACID PHOSPHATASE TYPE 5 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAATGCCGAGGCCAACGATGAGTTTCTTTCGCTTGAGTTGTAGTTCAT |
| Internal bar code: | GAACTACTTTAGAGGGTAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1965 |
| LEAP-Seq percent confirming: | 97.4359 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAACGGTACACTGCGTTA |
| Suggested primer 2: | GTCCTTGACGCAAAGGGGTA |