| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.014666 |
| Chromosome: | chromosome 1 |
| Location: | 5650879 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g040379 | (1 of 5) K14326 - regulator of nonsense transcripts 1 (UPF1, RENT1) | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAGCTCCTGCTTGAGGAAACGGATGAACTGCACGATGGTGGTGGTCTT |
| Internal bar code: | TTTGGTCGTATCTAATAGGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2447 |
| LEAP-Seq percent confirming: | 97.2222 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATGAAGAGGACGAGGACG |
| Suggested primer 2: | TAACCCATCCCCATCCCCAT |