Insertion cassette: | CIB2 |
Side of cassette: | 3' truncated? |
Strand: | - |
Strain: | CLIP2.014666 |
Chromosome: | chromosome 1 |
Location: | 5650882 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g040379 | (1 of 5) K14326 - regulator of nonsense transcripts 1 (UPF1, RENT1) | CDS/intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGTGGGTACTGGCGGGGCGGGGCGGGGCGGGGCTACCTCGGCGGTATG |
Internal bar code: | GGGTGATAGACAATTAAAGAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 728 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 17 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TAACCCATCCCCATCCCCAT |
Suggested primer 2: | CGATGAAGAGGACGAGGACG |