Insertion junction: CLIP2.014961_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.014961
Chromosome:chromosome 6
Location:1000511
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g256700 (1 of 1) IPR014001//IPR026127//IPR027417 - Helicase superfamily 1/2, ATP-binding domain // Probable RNA helicase SDE3 // P-loop containing nucleoside triphosphate hydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGCATCGGACCCTACCCCAATTCTTCGGGTGAGGGATACTCGACTGCTG
Internal bar code:CAGTAACGCTACTCTGGCAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:4640
LEAP-Seq percent confirming:65.625
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGTTCAACCGTGTTTCCG
Suggested primer 2:TGACGCCTCAGAGTGTCCTA