Insertion junction: CLIP2.015162_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.015162
Chromosome:chromosome 6
Location:3341871
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g277801 DNL4,DNL7 DNA ligase; (1 of 2) K10777 - DNA ligase 4 (LIG4, DNL4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACACACACACACACACACACACACACACACACACACACACACACA
Internal bar code:TCATCGGAAAGAGCGGACCATT

Confirmation - LEAP-Seq

LEAP-Seq distance:1261
LEAP-Seq percent confirming:91.6667
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TTACCACTCTTGCCACCACC
Suggested primer 2:GCTGCAACTGTACCTGCCTA