| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.015246 |
| Chromosome: | chromosome 9 |
| Location: | 6374307 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g414416 | CYCD4 | D-type cyclin; (1 of 1) IPR006671//IPR013763//IPR027417 - Cyclin, N-terminal // Cyclin-like // P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGTCCGTACTATTGTATGTTCGCTGGCTCGGGAGCTATGTCGCCCTTT |
| Internal bar code: | TACCAAAGCAGAGAGTCTTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2845 |
| LEAP-Seq percent confirming: | 94.5946 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGTTGGGTTGGTTAACAC |
| Suggested primer 2: | GGTTCGTGGGGTGGATTGAT |