Insertion junction: CLIP2.015246_4

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.015246
Chromosome:chromosome 12
Location:7817520
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g553900 CNG2 (1 of 1) K04905 - potassium voltage-gated channel Eag-related subfamily H member 2 (KCNH2); Cyclic-nucleotide gated potassium channel intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCACCATGTAGTTTGCCGTGATGGCGCTGGAGTCGCGCACCAGCTCGC
Internal bar code:CTCTAGTTCTACGCTAGGTGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1085
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGGTTGTAAGTACCAGCC
Suggested primer 2:CAATGCGGAAGATCTTGGCG