Insertion junction: CLIP2.015430_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.015430
Chromosome:chromosome 14
Location:517395
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g611300 COP3,CHR1,CSOA (1 of 7) PF01036 - Bacteriorhodopsin-like protein (Bac_rhodopsin); Channelrhodopsin 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAGGTCTGGTAGCCGTAGAACATCAGGCAGAGCGCTGAAAGCGCAAAA
Internal bar code:TGTTTAAGATCTTGGACGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:3925
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:65
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:65

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCTCACATGTGTGCCTTG
Suggested primer 2:TGACGAGGACGATACAGGGT