Insertion junction: CLIP2.015752_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.015752
Chromosome:chromosome 1
Location:458093
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g002787 SRR24A,SRR24 (1 of 1) PF00059//PF00530//PF00704//PF03762 - Lectin C-type domain (Lectin_C) // Scavenger receptor cysteine-rich domain (SRCR) // Glycosyl hydrolases family 18 (Glyco_hydro_18) // Vitelline membrane outer layer protein I (VOMI) (VOMI); Scavenger receptor cysteine-rich protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACACCTTGCGCTCTTTAACACGCACACACACACACACACACACACA
Internal bar code:TAGAAATTGTGGTCTATGGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1544
LEAP-Seq percent confirming:96.875
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTTGTCTCAAGCGTGTTG
Suggested primer 2:ATGTACCATCGGGATGACGC