| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.016402 |
| Chromosome: | chromosome 2 |
| Location: | 6423878 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388350 | MMP11,RAT2 | Metalloproteinase of VMP family; (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAACACGCAACAGCCCGTGCGGCGCAGCCGACTGCGCACCAGGTGCAGG |
| Internal bar code: | CGAGAGCTCAAATCAAAGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 701 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGCGTTTGTTTTCGAGTT |
| Suggested primer 2: | CCATGAAGGTGGAGGTGTCC |