Insertion junction: CLIP2.016421_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.016421
Chromosome:chromosome 14
Location:3781624
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g632050 POB25,RPG1,RPGRIP1 Retinitis pigmentosa GTPase regulator-interacting protein 1; (1 of 1) PF11618 - First C2 domain of RPGR-interacting protein 1 (C2-C2_1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACAATCGCCCCTGTCCTTCTTGTTCATCTCGGCTGCCGCCTCCTGCCCA
Internal bar code:ATTCTTCTACTGGGGTGAGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1072
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCACCTCTTCTTGAAGGT
Suggested primer 2:ACCATTCCCAGTAAGCAGCC