Insertion junction: CLIP2.016478_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.016478
Chromosome:chromosome 1
Location:460641
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g002787 SRR24A,SRR24 (1 of 1) PF00059//PF00530//PF00704//PF03762 - Lectin C-type domain (Lectin_C) // Scavenger receptor cysteine-rich domain (SRCR) // Glycosyl hydrolases family 18 (Glyco_hydro_18) // Vitelline membrane outer layer protein I (VOMI) (VOMI); Scavenger receptor cysteine-rich protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTAGGAAGAAGAACAGTCACTTGAACAAGACGCGCATGCCTTGTGACACA
Internal bar code:TAGACACTTTGGTTATAGAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:3524
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CCATGGCATGATGACTTGCG
Suggested primer 2:ACGGTGGCGTCATACTCATC