Insertion junction: CLIP2.016641_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.016641
Chromosome:chromosome 6
Location:945260
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g256350 (1 of 3) 1.1.2.6 - Polyvinyl alcohol dehydrogenase (cytochrome) / PVADH intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAACTCTTGGCAGCAGGCTCTGTTCAAAGTGGACACCGGCGCACAAGAC
Internal bar code:AAGACGTGACTAAACGGGATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:5486
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:90
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:90

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGGGTTTCGCAAACTTGG
Suggested primer 2:ACAAGAACTGGAGGAGTGCG