Insertion junction: CLIP2.016699_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.016699
Chromosome:chromosome 6
Location:4058642
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278221 TSSP1,TPS1,TSPSP1 (1 of 1) 2.4.1.15//3.1.3.12 - Alpha,alpha-trehalose-phosphate synthase (UDP-forming) / UDP-glucose--glucose-phosphate glucosyltransferase // Trehalose-phosphatase / Trehalose 6-phosphate phosphatase; Trehalose-6-phosphate synthase/phosphatase, class I intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGAAACCAACCCTGCCGGAACCGGCCCCCGCCAAATCCTTACCTTTGTA
Internal bar code:TAAATTTATTTAGCACAAGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2046
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:48
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:GGCAAAGGCAAGTGAACTGG
Suggested primer 2:TGATGGAGACCGTGTGTGTG