| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.016748 |
| Chromosome: | chromosome 17 |
| Location: | 2181038 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g712400 | TERC1,TERC | Thylakoid protein integration factor; (1 of 1) PTHR30238:SF0 - PROTEIN PIGMENT DEFECTIVE 149 | CDS |
| Cre17.g712450 | (1 of 2) PF08585 - RecQ mediated genome instability protein (RMI1_N) | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGATTCCATCCCCGCCGTCTTTGGTGTCACGCTCGACCCCTTCATCGTC |
| Internal bar code: | TGGACAGTGTGTGAGTTGTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2660 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTCAGCTTCAGTCCATGC |
| Suggested primer 2: | TGTTCGTCTTCGTCCTGGTG |