Insertion junction: CLIP2.016883_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.016883
Chromosome:chromosome 13
Location:2256154
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g578501 (1 of 1) IPR009060//IPR015940//IPR026741//IPR026937//IPR027417 - UBA-like // Ubiquitin-associated domain // Protein strawberry notch // Strawberry notch, helicase C domain // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCTTCCGCTCCCCCATTCCCCGTTTCAATATATCGCCTTCGTTTCTGA
Internal bar code:CGGATGTTATACGATGAAGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:260
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:TCTCCATGGCCAACAGTTCC
Suggested primer 2:GTACTGGACGAGTGCCACAA