| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.017503 |
| Chromosome: | chromosome 10 |
| Location: | 5895337 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g460750 | (1 of 1) PF00429 - ENV polyprotein (coat polyprotein) (TLV_coat) | intron | |
| Cre10.g460800 | (1 of 7) 2.7.11.1//2.7.11.17 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Calcium/calmodulin-dependent protein kinase / Microtubule-associated protein 2 kinase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTATTAATTGCTATTCCCTATTGTAGAAAAATAGGCGGCAGGGAAAACT |
| Internal bar code: | GACGGTGTCGGATTTAAAGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5201 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTGCAGCAGTTGTTTGAC |
| Suggested primer 2: | TGTGTCTCTGCATGTCGCTT |