Insertion junction: CLIP2.018092_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.018092
Chromosome:chromosome 12
Location:5591536
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g531150 XYI1 (1 of 1) 2.7.1.15//5.3.1.5 - Ribokinase // Xylose isomerase / D-xylose ketoisomerase; ribokinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGTCACGGCCCCTGACCCCCGAATCGCATCCCCCACGAAGTCCTGTGC
Internal bar code:GGTAAAATGGTTACTGTCCAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1052
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GATATGCCGAAAACACCGCC
Suggested primer 2:CCAGCCTGTGAGTGCCATAA