Insertion junction: CLIP2.018969_4

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.018969
Chromosome:chromosome 16
Location:5482891
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g682550 (1 of 1) PF08016//PF08263//PF13855 - Polycystin cation channel (PKD_channel) // Leucine rich repeat N-terminal domain (LRRNT_2) // Leucine rich repeat (LRR_8) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGTTCGCAACAGGAGGAAGTGCCCATAGGCAATGAGTTGACCTCAGTG
Internal bar code:GAAGAGGTCAATAGCTTTTTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:3228
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:GCTATGCAAATGTGGGCGAG
Suggested primer 2:GTCCTTACGATAGGTGCCCG