Insertion junction: CLIP2.019064_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.019064
Chromosome:chromosome 4
Location:3145761
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g226050 ARS10 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGATCCCAAGTACGACAGCCTGTACGCCGGATTCAAGAAGCTCGACTTC
Internal bar code:CCATACTAAATAGCACATTACC

Confirmation - LEAP-Seq

LEAP-Seq distance:2835
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:CCGGCCTTACAAACCCTTCT
Suggested primer 2:ATACCCCCTCTTCGCTGAGT