| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.019134 |
| Chromosome: | chromosome 11 |
| Location: | 2427076 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095121 | PUS9 | (1 of 1) PTHR21600//PTHR21600:SF11 - RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE B // PSEUDOURIDINE SYNTHASE FAMILY PROTEIN; RNA pseudouridine synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGCCCCGTGTCCCCATGTGACCAGTCGTGCCCGCCTCCTCCCTTCCC |
| Internal bar code: | TGACGGCGGGACCGGGTTTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 677 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGACAGAGGGACACGATGA |
| Suggested primer 2: | GCGGGTGTTAGAGATGGAGG |