| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.019799 |
| Chromosome: | chromosome 1 |
| Location: | 3820358 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g024950 | (1 of 1) PTHR12444//PTHR12444:SF0 - UNCHARACTERIZED // PROTEIN EFR3 HOMOLOG CMP44E | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCATGCGGTGCACCCTGCCAACCCCGCTCGCCAGTGCTACATGCATATT |
| Internal bar code: | CCAACCATGTTCAGGTGGATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4166 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAACCGCGAAAGGTCTAT |
| Suggested primer 2: | CCACATTTCGCTAGGCATGC |