Insertion junction: CLIP2.019876_4

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.019876
Chromosome:chromosome 12
Location:5313229
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g528300 5'UTR
Cre12.g528350 (1 of 1) IPR000408//IPR001810//IPR009091 - Regulator of chromosome condensation, RCC1 // F-box domain // Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTACAGAAATCGGGTCGCCGTCTTCTTTAGCTCAGAGGTCTCCAGCGT
Internal bar code:GTGGTTCCCGCCATGGTGACGT

Confirmation - LEAP-Seq

LEAP-Seq distance:3821
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:73
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:73

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACGAGGCGCAATAAGA
Suggested primer 2:AGAACTCCAGATTGCGCACA