Insertion junction: CLIP2.019920_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.019920
Chromosome:chromosome 4
Location:680096
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g217951 LCI33 Low-CO2-inducible protein; (1 of 9) IPR011011//IPR013083 - Zinc finger, FYVE/PHD-type // Zinc finger, RING/FYVE/PHD-type 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTTATCAAGGTCTTTGCTGAGCCCTACACGCGTCTTATCACACAACAT
Internal bar code:CTTCTAAAACTTTTTCGACGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:3632
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:74
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:74

Suggested primers for confirmation by PCR

Suggested primer 1:ACGCAGGTGCTTTTTCGTTC
Suggested primer 2:GGAGTCGGCGTATTACTGGG