Insertion junction: CLIP2.020572_4

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.020572
Chromosome:chromosome 13
Location:2231804
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g578350 MPA11 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 3) 3.1.3.26 - 4-phytase / Phytate 6-phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCTGCCTGCCTTTCCGTATCTCCCTAACTCAACCGTGCACGCCTCCCT
Internal bar code:ATAGGATTCGTTTGGGGTTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:1982
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:94
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:94

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAACTCACTGCTGGTTGG
Suggested primer 2:CACACTGGATCGGTGGTCAA