| Insertion cassette: | CIB2 | 
| Side of cassette: | 5' | 
| Strand: | + | 
| Strain: | CLIP2.020572 | 
| Chromosome: | chromosome 13 | 
| Location: | 2231804 | 
| Confidence (%): | 80 | 
| Locus systematic id | Locus common name | Defline | Feature | 
|---|---|---|---|
| Cre13.g578350 | MPA11 | Metallophosphoesterase/metallo-dependent phosphatase; (1 of 3) 3.1.3.26 - 4-phytase / Phytate 6-phosphatase | intron | 
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTGCCTGCCTTTCCGTATCTCCCTAACTCAACCGTGCACGCCTCCCT | 
| Internal bar code: | ATAGGATTCGTTTGGGGTTACG | 
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1982 | 
| LEAP-Seq percent confirming: | 100.0 | 
| LEAP-Seq n confirming: | 94 | 
| LEAP-Seq n nonconfirming: | 0 | 
| LEAP-Seq n unique pos: | 94 | 
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAACTCACTGCTGGTTGG | 
| Suggested primer 2: | CACACTGGATCGGTGGTCAA |