| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.020581 |
| Chromosome: | chromosome 16 |
| Location: | 3660043 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677026 | PDH1 | Pyruvate dehydrogenase E1 beta subunit; (1 of 2) K00162 - pyruvate dehydrogenase E1 component beta subunit (PDHB, pdhB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGGAACGCGCTGACACTTGGCCCTCTCGCAGGCGGGTTTCACGGGTAT |
| Internal bar code: | CGTAGTCATTATCGAGCCCTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2046 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGATTCTGATTCGTGCACC |
| Suggested primer 2: | TGCTCTTGACACGCGTAGTT |