| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.021286 |
| Chromosome: | chromosome 2 |
| Location: | 7350301 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391801 | VSP4 | (1 of 8) PTHR11339:SF290 - PROTEIN T26A8.1; Hydroxyproline-rich cell wall glycoprotein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAATGCCACTATCGACCCCAACGTGCTGCGCATCTATGGTACGTTGTAC |
| Internal bar code: | GGAAATCAAAGCGCGATTGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2354 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGCAGCCCGCTTTTGATA |
| Suggested primer 2: | ACTAGTATGCTGCGAGCGTC |