| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.021295 |
| Chromosome: | chromosome 3 |
| Location: | 6502504 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193800 | TSN1 | Aspartyl-tRNA synthetase; (1 of 1) 6.1.1.22 - Asparagine--tRNA ligase / Asparaginyl-tRNA synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTACCCCGGGTGCAGTCGGAGCCCTCCCCGCACCGCCCTGCTGTCCCCA |
| Internal bar code: | CAATGCCCTAACCACACGTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3244 |
| LEAP-Seq percent confirming: | 95.6522 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGGCATGAAGTCCCCATT |
| Suggested primer 2: | TGTTGCAATCGTGTCCCGTA |