| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.021599 |
| Chromosome: | chromosome 7 |
| Location: | 2960307 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g332950 | SFA,SFA1 | (1 of 1) PF06705 - SF-assemblin/beta giardin (SF-assemblin); SF-assemblin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGGGCAAACCGGCACTTACACAACAGGCCAGGCACACACATGTCGCGC |
| Internal bar code: | AGTCACTCTGGCTAAAAGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3267 |
| LEAP-Seq percent confirming: | 99.359 |
| LEAP-Seq n confirming: | 155 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 156 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCACGAGTTAAGCTGAGCA |
| Suggested primer 2: | ACACAACGAGATGAAGCCGT |