Insertion junction: CLIP2.022323_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.022323
Chromosome:chromosome 10
Location:1826631
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g430900 (1 of 1) IPR000104//IPR007259 - Antifreeze protein, type I // Gamma-tubulin complex component protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCTCCAGCTCCACCAGGCTGGTGCGCAGCACGTAGATGCAGGAGCTCA
Internal bar code:TGCACGTGCGCATTCGATCAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3754
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:GGATTGGTTGTGACGTGTGC
Suggested primer 2:CCCACCCACATACCGATACG