Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.022330 |
Chromosome: | chromosome 13 |
Location: | 1616451 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g573450 | CPS | Putative cleavage and polyadenylation specificity factor subunit 1; (1 of 1) K14401 - cleavage and polyadenylation specificity factor subunit 1 (CPSF1, CFT1) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGCCGCACCTCCGTCTATATCAACACTGCTGCGAGCCGGCAGCTGGCC |
Internal bar code: | CCGGCACAGTACCACAGCCGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2058 |
LEAP-Seq percent confirming: | 25.9259 |
LEAP-Seq n confirming: | 14 |
LEAP-Seq n nonconfirming: | 40 |
LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCTCGATGGGCATGTATGA |
Suggested primer 2: | GCCTTCCTGCTCATCACCAT |