Insertion junction: CLIP2.022552_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.022552
Chromosome:chromosome 1
Location:5417190
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g038151 (1 of 1) PTHR23069:SF0 - TAT-BINDING HOMOLOG 7 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCAGTTTCGCCTGGTTTGACTCACGGTAGTTTGGGCTGCTATTTACAAC
Internal bar code:CAGTAATTCTTCTTCGGTTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:208
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TGGAACCTGTCGAACAGCTC
Suggested primer 2:CTCCCACACACAGATGGTCC