| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.022672 |
| Chromosome: | chromosome 2 |
| Location: | 6433467 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388400 | RAT1 | (1 of 1) PF07798 - Protein of unknown function (DUF1640) (DUF1640); Chloroplast tscA maturation factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACAAGATGAAGGCCGAGATACGGTGCGAGGGAGCGCGGCGCCAGGGCA |
| Internal bar code: | ATGGGGACCGTTAGAGGACCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1777 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGGTCCGTGTACTTGTC |
| Suggested primer 2: | TGCCTCCTCTCCATAGTGCT |