Insertion junction: CLIP2.022889_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.022889
Chromosome:chromosome 2
Location:130071
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g074150 HKR1,COP5 (1 of 1) IPR001054//IPR001425//IPR001789//IPR003594//IPR003661//IPR004358//IPR005467//IPR011006//IPR013761//IPR029730//IPR029787 - Adenylyl cyclase class-3/4/guanylyl cyclase // Archaeal/bacterial/fungal rhodopsin // Signal transduction response regulator, receiver domain // Histidine kinase-like ATPase, C-terminal domain // Signal transduction histidine kinase, dimerisation/phosphoacceptor domain // Signal transduction histidine kinase-related protein, C-terminal // Histidine kinase domain // CheY-like superfamily // Sterile alpha motif/pointed domain // Archaeal/bacterial/fungal rhodopsin-like // Nucleotide cyclase; Histidine kinase rhodopsin 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTCATCACGCTGCTGTTCGAGCGCGAGAGCGCCAAGTTTCAGCTGGCG
Internal bar code:GCGACCCATGAAATACTCACAT

Confirmation - LEAP-Seq

LEAP-Seq distance:752
LEAP-Seq percent confirming:42.8571
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GACCTTGTTGTACGCCAGGA
Suggested primer 2:GCAGGACAGGATAGACGCTC