| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.022945 |
| Chromosome: | chromosome 6 |
| Location: | 5174950 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g283500 | POB3 | Proteome of basal body 3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGAGAGGACATGGGCGCGATGCCCTCCAAGCTGGCGTGTGAGCCGGC |
| Internal bar code: | TTCTGACAGAATTCATAATGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3568 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTCTTGCGCACCACATGT |
| Suggested primer 2: | GAGAGCAGGTAAGGATGGGC |