| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.023004 |
| Chromosome: | chromosome 7 |
| Location: | 3468954 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335750 | IFT88 | (1 of 1) K16474 - intraflagellar transport protein 88 (IFT88); Intraflagellar Transport Protein 88 | 3'UTR |
| Cre07.g800815 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAACGCAGGCTCGGCCCCGAACTCCGGGTGAGAGGCGGCGCGGCTGACA |
| Internal bar code: | GGAGGGAATCACATGAGCGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2276 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGTGTTTCGATCTCCCCA |
| Suggested primer 2: | ATGGCCCCATCTGTACTCCT |