| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.023173 |
| Chromosome: | chromosome 1 |
| Location: | 7156640 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g051250 | ODA-LC4,DLE1,DLC4 | Flagellar outer dynein arm 18 kDa light chain LC4; (1 of 1) PTHR23050//PTHR23050:SF195 - CALCIUM BINDING PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAGTAAGTGTATGCTGATGTCGATGCTAGGTTCAGCACTGCACTCTAC |
| Internal bar code: | CGTAGTGTCGTGCTGATTGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3429 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 90 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGATCGAGTCGCTGACAG |
| Suggested primer 2: | TCGCACTCCTCACCTCAAAC |