Insertion junction: CLIP2.023802_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.023802
Chromosome:chromosome 2
Location:6433204
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g388400 RAT1 (1 of 1) PF07798 - Protein of unknown function (DUF1640) (DUF1640); Chloroplast tscA maturation factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAAGAGCGAGCTTCAGAAGACCCAGGCAAGTGATGTGGCGCAGCGGTAG
Internal bar code:GAAGTCGGCGGCACGTGCGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:4444
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:66
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:66

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCTTGCCTCTGTCCAGTC
Suggested primer 2:TGCCTCCTCTCCATAGTGCT