Insertion junction: CLIP2.023969_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.023969
Chromosome:chromosome 6
Location:944974
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g256350 (1 of 3) 1.1.2.6 - Polyvinyl alcohol dehydrogenase (cytochrome) / PVADH intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTCTTCGTGTTACTGCTGGCGGTATGCAATGCGCGTGGCATGACGTACG
Internal bar code:CAACAATCAGAACCAGAAATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1802
LEAP-Seq percent confirming:93.1818
LEAP-Seq n confirming:41
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:GATCTTACCGGTGTTGCCCA
Suggested primer 2:CCAGGGTTTCGCAAACTTGG