| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.024905 |
| Chromosome: | chromosome 1 |
| Location: | 4040107 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026700 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCTACACCGGTCTGTTCACCACGTGCGGCTTCAGCCAGAGCAACTGT |
| Internal bar code: | CCGGTATGTGGACTCGGCCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3512 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGCAGCCCTATCCACAC |
| Suggested primer 2: | ACCTGCGTAACTTCACCCAG |