Insertion junction: CLIP2.025005_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.025005
Chromosome:chromosome 3
Location:3518425
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g167550 POC3,CEP290 Proteome of centriole protein 3; (1 of 1) K16533 - centrosomal protein CEP290 (CEP290, NPHP6) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCCACACGCATGCACGCACAGGGCCAAGGAGTCGCTGGCTCGCAACGC
Internal bar code:ACGTTACGTTTCCACGCTGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2422
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:58
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:58

Suggested primers for confirmation by PCR

Suggested primer 1:CACCTTCTCCTTGAGCAGCA
Suggested primer 2:CTCACATGCCAATCCCCCTT