Insertion junction: CLIP2.025343_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.025343
Chromosome:chromosome 1
Location:448270
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g002787 SRR24A,SRR24 (1 of 1) PF00059//PF00530//PF00704//PF03762 - Lectin C-type domain (Lectin_C) // Scavenger receptor cysteine-rich domain (SRCR) // Glycosyl hydrolases family 18 (Glyco_hydro_18) // Vitelline membrane outer layer protein I (VOMI) (VOMI); Scavenger receptor cysteine-rich protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTCTCCCTGCTCACTCTGGTCGGTCTCGCCCAGCGAGCGGTCGCCGCTG
Internal bar code:CTTTCGTCAAGGATACGTTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2205
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:CTACCTCGATCGTGTCACGG
Suggested primer 2:GCATGGGGAAGTACAGGCAT