| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.025353 |
| Chromosome: | chromosome 17 |
| Location: | 2324949 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713650 | MSH5 | DNA mismatch repair protein, MutS homolog; (1 of 1) K08741 - DNA mismatch repair protein MSH5 (MSH5) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCAGCTGGGTCCCGACCTGCTACTGCTCAGCAGCAAGTCAGACGCTGC |
| Internal bar code: | AATTGCAGGTCGAATGTTCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1270 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTTCCTCCTCCCCTTCCT |
| Suggested primer 2: | TGTGGTCTTGGCTAAGCTGG |