Insertion junction: CLIP2.026115_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.026115
Chromosome:chromosome 12
Location:1904489
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g511900 RPE1 Ribulose phosphate-3-epimerase, chloroplastic; (1 of 1) PTHR11749//PTHR11749:SF4 - RIBULOSE-5-PHOSPHATE-3-EPIMERASE // D-RIBULOSE-5-PHOSPHATE-3-EPIMERASE intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCATGCGCCACCACTGTCGGCCTGGGCCCAACCACTCCCGCGCGCGCG
Internal bar code:CCATTCCAGGGCGCAGCGGGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:3683
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:49
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:CCAACTTTTGTTACCGCGCA
Suggested primer 2:CGGTACCGATCCCTACTTGC