| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.026115 |
| Chromosome: | chromosome 12 |
| Location: | 1904510 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g511900 | RPE1 | Ribulose phosphate-3-epimerase, chloroplastic; (1 of 1) PTHR11749//PTHR11749:SF4 - RIBULOSE-5-PHOSPHATE-3-EPIMERASE // D-RIBULOSE-5-PHOSPHATE-3-EPIMERASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTGAGAGAGGCACAGTCATGATCTCTGCTTTGTGCATACACGGGTCC |
| Internal bar code: | CCATTCCAGGGCGCAGCGGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1429 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTACCGATCCCTACTTGC |
| Suggested primer 2: | CCAACTTTTGTTACCGCGCA |