| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.026140 |
| Chromosome: | chromosome 7 |
| Location: | 352409 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g314550 | TRM8,TMG9 | (1 of 1) PTHR23417//PTHR23417:SF16 - 3-DEOXY-D-MANNO-OCTULOSONIC-ACID TRANSFERASE/TRNA GUANINE-N 7 - -METHYLTRANSFERASE // TRNA (GUANINE-N(7)-)-METHYLTRANSFERASE; tRNA (guanine-N7)-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCAGCAGCAGCAGGGGGTGCGGGAGCAGAGGGAGCGGCATCAGGGGC |
| Internal bar code: | TCGTAGTTGAAGGGTAGATGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 78.125 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTTCACGTACCCAAGTGA |
| Suggested primer 2: | GTCATCCATGGCTTGGGGAA |